Human Aldolase A, Fructose Bisphosphate ELISA Kit | MyBioSource MBS724917 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Aldolase A, Fructose Bisphosphate ELISA Kit

catalog :

MBS724917

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS724917

products type :

ELISA Kit

products full name :

Human Aldolase A, Fructose Bisphosphate ELISA Kit

products short name :

Aldolase A, Fructose Bisphosphate

other names :

ALDOA, partial; Fructose-bisphosphate aldolase A; fructose-bisphosphate aldolase A; muscle-type aldolase; lung cancer antigen NY-LU-1; fructose-1,6-bisphosphate triosephosphate-lyase; epididymis secretory sperm binding protein Li 87p; aldolase A, fructose-bisphosphate; Lung cancer antigen NY-LU-1; Muscle-type aldolase

products gene name :

ALDOA

other gene names :

ALDOA; ALDOA; ALDA; GSD12; HEL-S-87p; ALDA

uniprot entry name :

ALDOA_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ALDOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALDOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: ALDOA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALDOA. Standards or samples are then added to the microtiter plate wells and ALDOA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALDOA present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ALDOA are added to each well to "sandwich" the ALDOA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALDOA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDOA concentration in each sample is interpolated from this standard curve

ncbi gi num :

49456715

ncbi acc num :

CAG46678.1

uniprot acc num :

P04075

ncbi mol weight :

45,261 Da

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

ncbi summary :

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

uniprot summary :

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate - glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Carbohydrate Metabolism - pentose phosphate pathway; Lyase; Carbohydrate Metabolism - fructose and mannose; Mitochondrial. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: I band; extracellular space; membrane; extracellular region; M band; nucleus; cytosol; actin cytoskeleton. Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding. Biological Process: platelet activation; striated muscle contraction; glycolysis; glucose metabolic process; actin filament organization; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; fructose 1,6-bisphosphate metabolic process; regulation of cell shape; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process. Disease: Glycogen Storage Disease Xii

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage