catalog number :
MBS724916
products type :
ELISA Kit
products full name :
Human Galanin ELISA Kit
products short name :
Galanin
other names :
galanin, GAL
specificity :
This assay has high sensitivity and excellent specificity for detection of GAL. No significant cross-reactivity or interference between GAL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAL and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL. Assay Type: Competitive
other info2 :
Intended Uses: This GAL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GAL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: GAL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAL antibody and an GAL-HRP conjugate. The assay sample and buffer are incubated together with GAL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL concentration since GAL from samples and GAL-HRP conjugate compete for the anti-GAL antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL from the sample, fewer sites are left to bind GAL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB34641.1