Human ATP binding cassette sub family A member 7 (ABCA7) ELISA Kit | MyBioSource MBS7249135 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ATP binding cassette sub family A member 7 (ABCA7) ELISA Kit

catalog :

MBS7249135

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7249135

products type :

ELISA Kit

products full name :

Human ATP binding cassette sub family A member 7 (ABCA7) ELISA Kit

products short name :

ATP binding cassette sub family A member 7 (ABCA7)

other names :

ATP-binding cassette sub-family A member 7; ATP-binding cassette sub-family A member 7; ATP-binding cassette sub-family A member 7; autoantigen SS-N; macrophage ABC transporter; ATP-binding cassette, sub-family A (ABC1), member 7; ABCA-SSN; Autoantigen SS-N; Macrophage ABC transporter

products gene name :

ABCA7

other gene names :

ABCA7; ABCA7; ABCX; ABCA-SSN

uniprot entry name :

ABCA7_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ABCA7. No significant cross-reactivity or interference between ABCA7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ABCA7 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This ABCA7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ABCA7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: ABCA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCA7 antibody and an ABCA7-HRP conjugate. The assay sample and buffer are incubated together with ABCA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCA7 concentration since ABCA7 from samples and ABCA7-HRP conjugate compete for the anti-ABCA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCA7 from the sample, fewer sites are left to bind ABCA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCA7 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

150417984

ncbi acc num :

NP_061985.2

ncbi gb acc num :

NM_019112.3

ncbi mol weight :

218,497 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); Transmembrane Transport Of Small Molecules Pathway (106572)

ncbi summary :

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]

uniprot summary :

ABCA7: Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Belongs to the ABC transporter superfamily. ABCA family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: Golgi membrane; Golgi apparatus; cell surface; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; ATP-binding cassette (ABC) transporter complex; phagocytic cup. Molecular Function: apolipoprotein A-I receptor activity; phospholipid transporter activity; transporter activity; ATPase activity; ATP binding. Biological Process: phospholipid efflux; positive regulation of ATPase activity; positive regulation of phagocytosis; negative regulation of amyloid precursor protein biosynthetic process; cholesterol efflux; peptide cross-linking; phospholipid scrambling; negative regulation of ATPase activity; transmembrane transport; phagocytosis; memory

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage