catalog number :
MBS724835
products type :
ELISA Kit
products full name :
Mouse RegeneRating Islet Derived Protein 1 Alpha ELISA Kit
products short name :
RegeneRating Islet Derived Protein 1 Alpha
products gene name :
REG1alpha
specificity :
This assay has high sensitivity and excellent specificity for detection of REG1alpha. No significant cross-reactivity or interference between REG1alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between REG1alpha and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Cell Biology
products description :
Intended Uses: This REG1alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse REG1alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay REG1alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-REG1alpha antibody and an REG1alpha-HRP conjugate. The assay sample and buffer are incubated together with REG1alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG1alpha concentration since REG1alpha from samples and REG1alpha-HRP conjugate compete for the anti-REG1alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by REG1alpha from the sample, fewer sites are left to bind REG1alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG1alpha concentration in each sample is interpolated from this standard curve.