ELISA/assay

Human Actin related protein 2/3 complex subunit 4 (ARPC4) ELISA Kit

MBS7248167

48-Strip-Wells

440 USD

ELISA Kit

Human Actin related protein 2/3 complex subunit 4 (ARPC4) ELISA Kit

[Actin related protein 2/3 complex subunit 4 (ARPC4)]

[Actin-related protein 2/3 complex subunit 4; Actin-related protein 2/3 complex subunit 4; ARPC4-TTLL3 fusion protein; ARPC4-TTLL3 read-through transcript; ARPC4-TTLL3 readthrough; Arp2/3 complex 20 kDa subunit; p20-ARC]

[ARPC4)]

[ARPC4-TTLL3; ARPC4; ARC20; p20-ARC]

ARPC4_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ARPC4. No significant cross-reactivity or interference between ARPC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARPC4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Signal Transduction

Intended Uses: This ARPC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARPC4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay ARPC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARPC4 antibody and an ARPC4-HRP conjugate. The assay sample and buffer are incubated together with ARPC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARPC4 concentration since ARPC4 from samples and ARPC4-HRP conjugate compete for the anti-ARPC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARPC4 from the sample, fewer sites are left to bind ARPC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARPC4 concentration in each sample is interpolated from this standard curve.

38372625

P59998.3

9,552 Da

CDC42 Signaling Events Pathway (137994); ErbB1 Downstream Signaling Pathway (138057); RAC1 Signaling Pathway (169355)

This locus represents naturally occurring read-through transcription between the neighboring ARPC4 (actin related protein 2/3 complex, subunit 4) and TTLL3 (tubulin tyrosine ligase-like family, member 3) genes. The read-through transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Nov 2010]

ARPC4: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. Belongs to the ARPC4 family. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p25.3. Cellular Component: Arp2/3 protein complex; cell projection; cytosol. Molecular Function: actin filament binding; protein binding, bridging; protein binding; enzyme binding; structural constituent of cytoskeleton. Biological Process: axon guidance; actin filament polymerization; ephrin receptor signaling pathway; innate immune response; actin nucleation

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415