ELISA/assay

Rat Interferon alpha ELISA Kit

MBS724814

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Interferon alpha ELISA Kit

Interferon alpha

Rat Interferon a ELISA Kit

interferon-alpha; Interferon alpha-8; interferon alpha-8; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B'; interferon alpha-B2; interferon alpha type 201; interferon, alpha 8; Interferon alpha-B; LeIF B; Interferon alpha-B2

IFN-alpha

IFN-a

IFNA8; IFNA8; IFN-alphaB; IFN-alpha-8; LeIF B

IFNA8_HUMAN

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This IFNalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IFNalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: IFNalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNalpha antibody and an IFNalpha-HRP conjugate. The assay sample and buffer are incubated together with IFNalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNalpha concentration since IFNalpha from samples and IFNalpha-HRP conjugate compete for the anti-IFNalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNalpha from the sample, fewer sites are left to bind IFNalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNalpha concentration in each sample is interpolated from this standard curve.

306902

AAA52716.1

21,989 Da

Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)

IFNA8: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675