Human F box only protein 32 (FBXO32) ELISA Kit | MyBioSource MBS7247935 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human F box only protein 32 (FBXO32) ELISA Kit

catalog :

MBS7247935

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7247935

products type :

ELISA Kit

products full name :

Human F box only protein 32 (FBXO32) ELISA Kit

products short name :

[F box only protein 32 (FBXO32)]

other names :

[F-box only protein 32 isoform 3; F-box only protein 32; F-box only protein 32; atrogin 1; atrogin-1; muscle atrophy F-box protein; F-box protein 32; Atrogin-1; Muscle atrophy F-box protein; MAFbx]

products gene name :

[FBXO32]

other gene names :

[FBXO32; FBXO32; Fbx32; MAFbx; MAFbx]

uniprot entry name :

FBX32_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of FBN. No significant cross-reactivity or interference between FBN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBN and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

products categories :

Cell Biology

products description :

Intended Uses: This FBN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine FBN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FBN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FBN antibody and an FBN-HRP conjugate. The assay sample and buffer are incubated together with FBN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBN concentration since FBN from samples and FBN-HRP conjugate compete for the anti-FBN antibody binding site. Since the number of sites is limited, as more sites are occupied by FBN from the sample, fewer sites are left to bind FBN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBN concentration in each sample is interpolated from this standard curve.

ncbi gi num :

335057520

ncbi acc num :

NP_001229392.1

ncbi gb acc num :

NM_001242463.1

ncbi mol weight :

30,804 Da

ncbi pathways :

FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Monoamine Transport Pathway (198851)

ncbi summary :

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

uniprot summary :

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 8q24.13. Cellular Component: nucleolus; Z disc. Molecular Function: protein binding. Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage