ELISA/assay

Human Anti Type II Collagen Antibody ELISA Kit

MBS724782

48-Strip-Wells

470 USD

ELISA Kit

Human Anti Type II Collagen Antibody ELISA Kit

Anti Type II Collagen Antibody

type II collagen, partial; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; arthroophthalmopathy, progressive (Stickler syndrome); collagen, type II, alpha 1; Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin

CII

COL2A1; COL2A1; AOM; ANFH; SEDC; STL1; COL11A3

CO2A1_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This 5 - HIAA ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of 5 - HIAA in the sample, this 5 - HIAA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus 5 - HIAA concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of 5 - HIAA utilizes a multiclonal anti-5 - HIAA antibody and an 5 - HIAA-HRP conjugate. The assay sample and buffer are incubated together with 5 - HIAA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5 - HIAA concentration since 5 - HIAA from samples and 5 - HIAA-HRP conjugate compete for the anti-5 - HIAA antibody binding site. Since the number of sites is limited, as more sites are occupied by 5 - HIAA from the sample, fewer sites are left to bind 5 - HIAA-HRP conjugate. Standards of known 5 - HIAA concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of 5 - HIAA. The 5 - HIAA concentration in each sample is interpolated from this standard curve.

180809

AAA52037.1

29,781 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795)

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12q13.11. Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane. Molecular Function: identical protein binding; metal ion binding; platelet-derived growth factor binding; extracellular matrix structural constituent conferring tensile strength. Biological Process: heart morphogenesis; proteoglycan metabolic process; axon guidance; inner ear morphogenesis; extracellular matrix organization and biogenesis; central nervous system development; collagen fibril organization; palate development; notochord development; extracellular matrix disassembly; limb bud formation; collagen catabolic process; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; cartilage condensation; endochondral ossification. Disease: Achondrogenesis, Type Ii; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Kniest Dysplasia; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Spondyloepiphyseal Dysplasia Congenita; Avascular Necrosis Of Femoral Head, Primary; Legg-calve-perthes Disease; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Spondyloperipheral Dysplasia; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Stickler Syndrome, Type I, Nonsyndromic Ocular

48-Strip-Wells

470 USD

96-Strip-Wells

675