ELISA/assay

Rat C-type lectin domain family 2 member D (CLEC2D) ELISA Kit

MBS7247696

48-Strip-Wells

470 USD

ELISA Kit

Rat C-type lectin domain family 2 member D (CLEC2D) ELISA Kit

C-type lectin domain family 2 member D (CLEC2D)

C-type lectin domain family 2 member D; C-type lectin domain family 2 member D; C-type lectin domain family 2 member D; osteoclast inhibitory lectin; C-type lectin-related protein B; lectin-like transmembrane protein; C-type lectin-domain family 2 member D; C-type lectin domain family 2, member d; C-type lectin-related protein B; Clr-b; Lectin-like transmembrane protein; Osteoclast inhibitory lectin

CLEC2D

Clec2d; Clec2d; Clrb; Ocil; Clr-b; Clrb; Ocil; Clr-b

CLC2D_MOUSE

Rat

This assay has high sensitivity and excellent specificity for detection of CLEC2D. No significant cross-reactivity or interference between CLEC2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC2D and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This CLEC2D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CLEC2D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CLEC2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC2D antibody and an CLEC2D-HRP conjugate. The assay sample and buffer are incubated together with CLEC2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC2D concentration since CLEC2D from samples and CLEC2D-HRP conjugate compete for the anti-CLEC2D antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC2D from the sample, fewer sites are left to bind CLEC2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC2D concentration in each sample is interpolated from this standard curve.

16716407

NP_444339.1

NM_053109.3

23,647 Da

CLEC2D: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon- gamma. Binds high molecular weight sulfated glycosaminoglycans. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding; natural killer cell lectin-like receptor binding. Biological Process: negative regulation of osteoclast differentiation; cellular defense response; signal transduction; protection from natural killer cell mediated cytotoxicity

48-Strip-Wells

470 USD

96-Strip-Wells

675