ELISA/assay

Mouse Prokineticin 2 ELISA Kit

MBS724741

48-Strip-Wells

470 USD

ELISA Kit

Mouse Prokineticin 2 ELISA Kit

Prokineticin 2

prokineticin-2 isoform a; Prokineticin-2; prokineticin-2; protein Bv8 homolog; prokineticin 2; Protein Bv8 homolog

PK2

PROK2; PROK2; BV8; HH4; PK2; KAL4; MIT1; BV8; PK2

PROK2_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: PK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PK2 antibody and an PK2-HRP conjugate. The assay sample and buffer are incubated together with PK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PK2 concentration since PK2 from samples and PK2-HRP conjugate compete for the anti-PK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PK2 from the sample, fewer sites are left to bind PK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PK2 concentration in each sample is interpolated from this standard curve.

187167261

NP_001119600.1

NM_001126128.1

11,659 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p13. Cellular Component: extracellular region. Molecular Function: G-protein-coupled receptor binding. Biological Process: circadian rhythm; activation of MAPK activity; sensory perception of pain; chemotaxis; G-protein coupled receptor protein signaling pathway; cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis. Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia

48-Strip-Wells

470 USD

96-Strip-Wells

675