ELISA/assay

Rat N-methyl-D-aspartate receptor 1 ELISA Kit

MBS724735

48-Strip-Wells

470 USD

ELISA Kit

Rat N-methyl-D-aspartate receptor 1 ELISA Kit

N-methyl-D-aspartate receptor 1

NMDA receptor 1; Glutamate [NMDA] receptor subunit 1; CG2902 gene product from transcript CG2902-RA; CG2902-PA; NMDA receptor; NMDA receptor 1; NMDA receptor I; NMDAR; Nmdar1-PA; dNR1; NMDA receptor 1

NMDA-R1

Nmdar1; Nmdar1; CG2902; DmelNmdar1; DmelCG2902; DMNMDAR1; DNmdar; dnmdar-I; DNMDAR-I; dNR1; DrNR1; dsNR1; NMDA; NMDA R1; Nmdar; NMdar1; NMDAR1; nmr; NR1; NRI; nmr; NR1; CG2902; DNMDAR-I; dNR1

NMDA1_DROME

Rat

This assay has high sensitivity and excellent specificity for detection of NMDAR1. No significant cross-reactivity or interference between NMDAR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NMDAR1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This NMDAR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NMDAR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. Principle of the Assay: NMDAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NMDAR1 antibody and an NMDAR1-HRP conjugate. The assay sample and buffer are incubated together with NMDAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NMDAR1 concentration since NMDAR1 from samples and NMDAR1-HRP conjugate compete for the anti-NMDAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NMDAR1 from the sample, fewer sites are left to bind NMDAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NMDAR1 concentration in each sample is interpolated from this standard curve.

24644257

NP_730940.1

NM_169059.2

112,288 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1006020); Axon Guidance Pathway (1005335); CREB Phosphorylation Through The Activation Of CaMKII Pathway (1006026); Developmental Biology Pathway (1005334); EPH-Ephrin Signaling Pathway (1005365); EPHB-mediated Forward Signaling Pathway (1005367); Neuroactive Ligand-receptor Interaction Pathway (119911); Neuroactive Ligand-receptor Interaction Pathway (462); Neuronal System Pathway (1005991); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (1006008)

48-Strip-Wells

470 USD

96-Strip-Wells

675