ELISA/assay

Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit

MBS724700

48-Strip-Wells

470 USD

ELISA Kit

Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit

Procollagen C Proteinase Enhancer 1

procollagen C-endopeptidase enhancer 1; Procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer

PCPE1

PCOLCE; PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1

PCOC1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of PCPE1. No significant cross-reactivity or interference between PCPE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCPE1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Principle of the assay: PCPE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCPE1 antibody and an PCPE1-HRP conjugate. The assay sample and buffer are incubated together with PCPE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCPE1 concentration since PCPE1 from samples and PCPE1-HRP conjugate compete for the anti-PCPE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCPE1 from the sample, fewer sites are left to bind PCPE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCPE1 concentration in each sample is interpolated from this standard curve.

157653329

NP_002584.2

NM_002593.3

47,972 Da

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Extracellular Matrix Organization Pathway (576262)

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Protein type: Secreted, signal peptide; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular matrix; extracellular space. Molecular Function: heparin binding; collagen binding; protein binding; protease activator activity. Biological Process: multicellular organismal development; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675