ELISA/assay

Rabbit Pulmonary Surfactant Associated Protein C ELISA Kit

MBS724694

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Pulmonary Surfactant Associated Protein C ELISA Kit

Pulmonary Surfactant Associated Protein C

pulmonary surfactant-associated protein C; Pulmonary surfactant-associated protein C; pulmonary surfactant-associated protein C; pulmonary surfactant-associated proteolipid SPL(Val); surfactant associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5

SPC

Sftpc; Sftpc; SP5; SPC; SP-C; Sftp2; Bricd6; Sftp-2; pro-SpC; Sftp2; SP-C

PSPC_MOUSE

Rabbit

This assay has high sensitivity and excellent specificity for detection of SPC. No significant cross-reactivity or interference between SPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This SPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit SPC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: SPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPC antibody and an SPC-HRP conjugate. The assay sample and buffer are incubated together with SPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPC concentration since SPC from samples and SPC-HRP conjugate compete for the anti-SPC antibody binding site. Since the number of sites is limited, as more sites are occupied by SPC from the sample, fewer sites are left to bind SPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPC concentration in each sample is interpolated from this standard curve.

256355065

NP_035489.2

NM_011359.2

21,055 Da

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called hyaline membranes . 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid-binding. Cellular Component: multivesicular body; extracellular space; cytoplasm; extracellular region. Molecular Function: protein homodimerization activity. Biological Process: protein homooligomerization

48-Strip-Wells

470 USD

96-Strip-Wells

675