catalog number :
MBS7246438
products type :
ELISA Kit
products full name :
Mouse C1q/TNF-related protein-3, Cartonectin/CTRP3/CORS-26 ELISA Kit
products short name :
C1q/TNF-related protein-3, Cartonectin/CTRP3/CORS-26
products gene name :
C1QTNF3
specificity :
This assay has high sensitivity and excellent specificity for detection of CTRP3. No significant cross-reactivity or interference between CTRP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTRP3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This CTRP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CTRP3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CTRP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTRP3 antibody and an CTRP3-HRP conjugate. The assay sample and buffer are incubated together with CTRP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTRP3 concentration since CTRP3 from samples and CTRP3-HRP conjugate compete for the anti-CTRP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTRP3 from the sample, fewer sites are left to bind CTRP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTRP3 concentration in each sample is interpolated from this standard curve.