Mouse C-type lectin domain family 4 member e, CLEC4E ELISA Kit | MyBioSource MBS7246171 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse C-type lectin domain family 4 member e, CLEC4E ELISA Kit

catalog :

MBS7246171

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7246171

products type :

ELISA Kit

products full name :

Mouse C-type lectin domain family 4 member e, CLEC4E ELISA Kit

products short name :

C-type lectin domain family 4 member e, CLEC4E

other names :

C-type lectin domain family 4 member E; C-type lectin domain family 4 member E; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; macrophage-inducible C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; C-type lectin domain family 4, member E; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin

products gene name :

CLEC4E

other gene names :

CLEC4E; CLEC4E; MINCLE; CLECSF9; CLECSF9; MINCLE; UNQ218/PRO244

uniprot entry name :

CLC4E_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of CLEC4E. No significant cross-reactivity or interference between CLEC4E and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC4E and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This CLEC4E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLEC4E. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: CLEC4E ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC4E antibody and an CLEC4E-HRP conjugate. The assay sample and buffer are incubated together with CLEC4E-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC4E concentration since CLEC4E from samples and CLEC4E-HRP conjugate compete for the anti-CLEC4E antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC4E from the sample, fewer sites are left to bind CLEC4E-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC4E concentration in each sample is interpolated from this standard curve.

ncbi gi num :

7657333

ncbi acc num :

NP_055173.1

ncbi gb acc num :

NM_014358.2

ncbi mol weight :

25,073 Da

ncbi pathways :

Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

uniprot summary :

CLEC4E: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6 -dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to membrane. Molecular Function: receptor activity; carbohydrate binding. Biological Process: immune response; positive regulation of cytokine secretion

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage