ELISA/assay

Human 14 3 3 protein eta (YWHAH) ELISA Kit

MBS7245863

48-Strip-Wells

470 USD

ELISA Kit

Human 14 3 3 protein eta (YWHAH) ELISA Kit

14 3 3 protein eta (YWHAH)

14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Protein AS1

YWHAH; YWHAH; YWHA1; YWHA1

1433F_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of YWHAH. No significant cross-reactivity or interference between YWHAH and analogues was observed. NOTE: cross-reactivity detection between YWHAH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses This YWHAH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YWHAH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: YWHAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAH antibody and an YWHAH-HRP conjugate. The assay sample and buffer are incubated together with YWHAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAH concentration since YWHAH from samples and YWHAH-HRP conjugate compete for the anti-YWHAH antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAH from the sample, fewer sites are left to bind YWHAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAH concentration in each sample is interpolated from this standard curve.

4507951

NP_003396.1

NM_003405.3

28,219 Da

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Epstein-Barr Virus Infection Pathway (585562)

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Nuclear receptor co-regulator; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 22q12.3. Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding. Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; glucocorticoid receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport

48-Strip-Wells

470 USD

96-Strip-Wells

675