ELISA/assay

Guinea pig Hepatoma Derived Growth Factor ELISA Kit

MBS724579

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Hepatoma Derived Growth Factor ELISA Kit

Hepatoma Derived Growth Factor

hepatoma-derived growth factor isoform a; Hepatoma-derived growth factor; hepatoma-derived growth factor; HMG-1L2; high mobility group protein 1-like 2; hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2

HDGF

HDGF; HDGF; HMG1L2; HMG1L2; HDGF; HMG-1L2

HDGF_HUMAN

Guinea Pig

This assay has high sensitivity and excellent specificity for detection of HDGF. No significant cross-reactivity or interference between HDGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HDGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This HDGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig HDGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: HDGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HDGF. Standards or samples are then added to the microtiter plate wells and HDGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HDGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HDGF are added to each well to "sandwich" the HDGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HDGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDGF concentration in each sample is interpolated from this standard curve.

4758516

NP_004485.1

NM_004494.2

28,264 Da

IRE1alpha Activates Chaperones Pathway (105906); Metabolism Of Proteins Pathway (106230); Unfolded Protein Response (UPR) Pathway (105904); XBP1(S) Activates Chaperone Genes Pathway (530771)

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family. Protein type: Transcription regulation; Cytokine. Chromosomal Location of Human Ortholog: 1q23.1. Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm. Molecular Function: heparin binding; DNA binding; growth factor activity; nucleotide binding. Biological Process: cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction

48-Strip-Wells

470 USD

96-Strip-Wells

675