ELISA/assay

Mouse Protein kinase C alpha ELISA Kit

MBS724560

48-Strip-Wells

470 USD

ELISA Kit

Mouse Protein kinase C alpha ELISA Kit

Protein kinase C alpha

protein kinase C alpha type; Protein kinase C alpha type; protein kinase C alpha type; PKC-A; aging-associated gene 6; protein kinase C, alpha

PKCa

PRKCA; PRKCA; AAG6; PKCA; PRKACA; PKC-alpha; PKCA; PRKACA; PKC-A; PKC-alpha

KPCA_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PKCa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PKCa. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: PKCa ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKCa antibody and an PKCa-HRP conjugate. The assay sample and buffer are incubated together with PKCa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKCa concentration since PKCa from samples and PKCa-HRP conjugate compete for the anti-PKCa antibody binding site. Since the number of sites is limited, as more sites are occupied by PKCa from the sample, fewer sites are left to bind PKCa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCa concentration in each sample is interpolated from this standard curve.

4506067

NP_002728.1

NM_002737.2

76,750 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Regulates Insulin Secretion Pathway (105924); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.13; Protein kinase, AGC; AGC group; PKC family; Alpha subfamily. Chromosomal Location of Human Ortholog: 17q22-q23.2. Cellular Component: nucleoplasm; photoreceptor outer segment; mitochondrion; cell soma; perinuclear region of cytoplasm; apical part of cell; endoplasmic reticulum; mitochondrial membrane; dendrite; cytoplasm; plasma membrane; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; mitotic nuclear envelope disassembly; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; synaptic transmission; negative regulation of cell proliferation; chondrocyte differentiation; angiogenesis; positive regulation of macrophage differentiation; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; platelet activation; neutrophil chemotaxis; fibroblast growth factor receptor signaling pathway; negative regulation of adenylate cyclase activity; adenylate cyclase activation; positive regulation of blood vessel endothelial cell migration; rhodopsin mediated signaling; cellular calcium ion homeostasis; positive regulation of angiogenesis; negative regulation of glucose import; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; gene expression; positive regulation of endothelial cell proliferation; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion; positive regulation of inflammatory response; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675