Human Cocaine esterase (CES2) ELISA Kit | MyBioSource MBS7245485 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cocaine esterase (CES2) ELISA Kit

catalog :

MBS7245485

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7245485

products type :

ELISA Kit

products full name :

Human Cocaine esterase (CES2) ELISA Kit

products short name :

Cocaine esterase (CES2)

other names :

cocaine esterase isoform 2; Cocaine esterase; cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2; carboxylesterase 2; Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56)

products gene name :

CES2

other gene names :

CES2; CES2; iCE; CE-2; PCE-2; CES2A1; ICE; CE-2; hCE-2

uniprot entry name :

EST2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CES2. No significant cross-reactivity or interference between CES2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CES2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CES2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CES2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: CES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CES2 antibody and an CES2-HRP conjugate. The assay sample and buffer are incubated together with CES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CES2 concentration since CES2 from samples and CES2-HRP conjugate compete for the anti-CES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CES2 from the sample, fewer sites are left to bind CES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CES2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

37622887

ncbi acc num :

NP_932327.1

ncbi gb acc num :

NM_198061.2

ncbi mol weight :

59,959 Da

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Irinotecan Pathway (198905); Phase I, Non P450 Pathway (198854); Retinol Biosynthesis Pathway (545293)

ncbi summary :

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]

uniprot summary :

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.84; EC 3.1.1.56; Xenobiotic Metabolism - drug metabolism - other enzymes; Endoplasmic reticulum; EC 3.1.1.1; Hydrolase. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum. Molecular Function: methylumbelliferyl-acetate deacetylase activity. Biological Process: catabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage