ELISA/assay

Rabbit Inducible nitric oxide synthase ELISA Kit

MBS724500

96 Strip Wells

675 USD

ELISA Kit

Rabbit Inducible nitric oxide synthase ELISA Kit

Inducible nitric oxide synthase

inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase

iNOS

Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; CG11143; MIP synthase; IPS; MI-1-P synthase

INO1_DROME

Rabbit

Store all reagents at 2-8 degree C.

Neurobiology

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This INOS ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of INOS in the sample, this INOS ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus INOS concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of INOS utilizes a multiclonal anti-INOS antibody and an INOS-HRP conjugate. The assay sample and buffer are incubated together with INOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INOS concentration since INOS from samples and INOS-HRP conjugate compete for the anti-INOS antibody binding site. Since the number of sites is limited, as more sites are occupied by INOS from the sample, fewer sites are left to bind INOS-HRP conjugate. Standards of known INOS concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of INOS. The INOS concentration in each sample is interpolated from this standard curve.

17137626

NP_477405.1

NM_058057.6

62,249 Da

Inositol Phosphate Metabolism Pathway 3024!!Inositol Phosphate Metabolism Pathway 1006205!!Inositol Phosphate Metabolism Pathway 362!!Metabolic Pathways 132021!!Metabolism Pathway 1006204!!Synthesis Of IP2, IP, And Ins In The Cytosol Pathway 1006210

96 Strip Wells

675 USD