ELISA/assay

Rat Metallothionein, MT ELISA Kit

MBS724498

48-Strip-Wells

470 USD

ELISA Kit

Rat Metallothionein, MT ELISA Kit

Metallothionein, MT

Rat Metallothionein, MT ELISA Kit; Metallothionein, MT; Metallothionein, MT ELISA Kit (Rat)

metallothionein; Metallothionein; metallothionein; MT; metallothionein 2A; metallothionein 4<

MT

MT4; MT2A

MT_CHICK

Rat

This assay has high sensitivity and excellent specificity for detection of MET. No significant cross-reactivity or interference between MET and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MET and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MET ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MET. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the Assay: MET ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MET antibody and an MET-HRP conjugate. The assay sample and buffer are incubated together with MET-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MET concentration since MET from samples and MET-HRP conjugate compete for the anti-MET antibody binding site. Since the number of sites is limited, as more sites are occupied by MET from the sample, fewer sites are left to bind MET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MET concentration in each sample is interpolated from this standard curve.

46048711

NP_990606.1

NM_205275.1

P68497

6,462 Da

Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals. Domain: Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. Sequence similarities: Belongs to the metallothionein superfamily. Type 1 family.

48-Strip-Wells

470 USD

96-Strip-Wells

675