catalog number :
MBS724497
products type :
ELISA Kit
products full name :
Mouse Arachidonic Acid ELISA Kit
products short name :
Arachidonic Acid
other names :
arachidonic acid epoxygenase, partial; Cytochrome P450 2C23; Arachidonic acid epoxygenase; CYPIIC23
other gene names :
Cyp2c23; Cyp2c-23
uniprot entry name :
CP2CN_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of AA. No significant cross-reactivity or interference between AA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.
products description :
Intended Uses: This AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the vvdetermination of Mouse AA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay AA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AA. Standards or samples are then added to the microtiter plate wells and AA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AA are added to each well to "sandwich" the AA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB29022.1
ncbi mol weight :
56,433 Da