catalog number :
MBS724464
products type :
ELISA Kit
products full name :
Mouse Serum amyloid A 3 ELISA Kit
products short name :
Serum amyloid A 3
other names :
serum amyloid A-3 protein; Serum amyloid A-3 protein; serum amyloid A-3 protein; serum amyloid A 3
products gene name :
SAA3
other gene names :
Saa3; Saa3; l7R3; Saa-3; AV098916
uniprot entry name :
SAA3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of SAA3. No significant cross-reactivity or interference between SAA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SAA3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mug/mL.
other info2 :
Intended Uses: This SAA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SAA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: SAA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAA3 antibody and an SAA3-HRP conjugate. The assay sample and buffer are incubated together with SAA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAA3 concentration since SAA3 from samples and SAA3-HRP conjugate compete for the anti-SAA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA3 from the sample, fewer sites are left to bind SAA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA3 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_035445.1
ncbi gb acc num :
NM_011315.3
ncbi mol weight :
13,774 Da
uniprot summary :
SAA3: . Cellular Component: extracellular space; extracellular region. Molecular Function: chemoattractant activity. Biological Process: positive chemotaxis; acute-phase response; I-kappaB phosphorylation