Human Cannabinoid receptor 2 (CNR2) ELISA Kit | MyBioSource MBS7244347 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cannabinoid receptor 2 (CNR2) ELISA Kit

catalog :

MBS7244347

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7244347

products type :

ELISA Kit

products full name :

Human Cannabinoid receptor 2 (CNR2) ELISA Kit

products short name :

[Cannabinoid receptor 2 (CNR2)]

other names :

[cannabinoid receptor 2; Cannabinoid receptor 2; cannabinoid receptor 2; testis-dominant CNR2 isoform CB2; cannabinoid receptor 2 (macrophage); CX5]

products gene name :

[CNR2]

other gene names :

[CNR2; CNR2; CB2; CX5; CB-2; CB2A; CB2B; CB-2; CB2; hCB2]

uniprot entry name :

CNR2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CNR2. No significant cross-reactivity or interference between CNR2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNR2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Neurobiology

products description :

Intended Uses: This CNR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CNR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNR2 antibody and an CNR2-HRP conjugate. The assay sample and buffer are incubated together with CNR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNR2 concentration since CNR2 from samples and CNR2-HRP conjugate compete for the anti-CNR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNR2 from the sample, fewer sites are left to bind CNR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNR2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4502929

ncbi acc num :

NP_001832.1

ncbi gb acc num :

NM_001841.2

ncbi mol weight :

39,681 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); Small Ligand GPCRs Pathway (198892)

ncbi summary :

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]

uniprot summary :

CNR2: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon. Molecular Function: cannabinoid receptor activity. Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; sensory perception of pain; inflammatory response; negative regulation of synaptic transmission, GABAergic

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage