ELISA/assay

Rat tenascin-R ELISA Kit

MBS724395

48-Strip-Wells

470 USD

ELISA Kit

Rat tenascin-R ELISA Kit

tenascin-R

Rat tenascin-R ELISA Kit; tenascin-R; tenascin-R (Rat)

tenascin-R; Tenascin-R; tenascin-R; janusin; restrictin; tenascin R (restrictin, janusin); tenascin R; Janusin; Restrictin

TN-R

TNR; TNR; TN-R; TN-R

TENR_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of TER. No significant cross-reactivity or interference between TER and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TER and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TER. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the assay: TER ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TER antibody and an TER-HRP conjugate. The assay sample and buffer are incubated together with TER-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TER concentration since TER from samples and TER-HRP conjugate compete for the anti-TER antibody binding site. Since the number of sites is limited, as more sites are occupied by TER from the sample, fewer sites are left to bind TER-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TER concentration in each sample is interpolated from this standard curve.

157384973

NP_003276.3

NM_003285.2

Q92752

149,562 Da

ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979)

Tenascin-R (TNR) is an extracellular matix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion; MIM 187380), TNX (TNXB; MIM 600985), and TNR (Erickson, 1993 [PubMed 7694605]). The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues.[supplied by OMIM, Mar 2008]

Function: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance . By similarity. Subunit structure: Forms oligomers. Interacts with CNTN1, TNC, and FN1. Interacts with BCAN and ACAN in a calcium-dependent manner. Interacts with SCN2B, PTPRZ1, and CSPG3 . By similarity. Subcellular location: Secreted extracellular space extracellular matrix. Tissue specificity: Brain specific. Ref.1. Domain: The EGF-like domains mediate interaction with CNTN1. The fibronectin type-III domains 3-5 mediate interaction with BCAN. The fibronectin type-III domains 1-2 and 7-9 mediate interaction with SCN2B . By similarity. Post-translational modification: Contains N-linked oligosaccharides, O-linked sialylated structures and O-linked chondroitin sulfate glycosaminoglycans. Contains N-linked oligosaccharides with a sulfated carbohydrate structure . By similarity. O-glycosylated on Thr-36 or Thr-37 with a core 1 or possibly core 8 glycan. Sequence similarities: Belongs to the tenascin family.Contains 5 EGF-like domains.Contains 1 fibrinogen C-terminal domain.Contains 9 fibronectin type-III domains.

48-Strip-Wells

470 USD

96-Strip-Wells

675