ELISA/assay

Mouse c-myc Oncogene product ELISA Kit

MBS724363

48-Strip-Wells

470 USD

ELISA Kit

Mouse c-myc Oncogene product ELISA Kit

c-myc Oncogene product

c-myc, partial; C-myc protein; myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor; v-myc avian myelocytomatosis viral oncogene homolog; C-myc protein

C-MYC

MYC; c-myc; MRTL; MYCC; c-Myc; bHLHe39

Q6LBK7_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CmycOP. No significant cross-reactivity or interference between CmycOP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CmycOP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CmycOP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CmycOP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

. Principle of the assay: CmycOP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CmycOP antibody and an CmycOP-HRP conjugate. The assay sample and buffer are incubated together with CmycOP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CmycOP concentration since CmycOP from samples and CmycOP-HRP conjugate compete for the anti-CmycOP antibody binding site. Since the number of sites is limited, as more sites are occupied by CmycOP from the sample, fewer sites are left to bind CmycOP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CmycOP concentration in each sample is interpolated from this standard curve.

396512

CAA46984.1

Q6LBK7

7,012 Da

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Apoptosis Pathway (198797); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); C-MYB Transcription Factor Network Pathway (138073); C-MYC Pathway (169344); CD40/CD40L Signaling Pathway (138061); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765)

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

48-Strip-Wells

470 USD

96-Strip-Wells

675