Rat Fibrillin 1 ELISA Kit | MyBioSource MBS724350 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Fibrillin 1 ELISA Kit

catalog :

MBS724350

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS724350

products type :

ELISA Kit

products full name :

Rat Fibrillin 1 ELISA Kit

products short name :

Fibrillin 1

other names :

fibrillin-1; Fibrillin-1; fibrillin-1; fibrillin 15; fibrillin 1

products gene name :

FBN1

other gene names :

FBN1; FBN1; FBN; SGS; WMS; MASS; MFS1; OCTD; SSKS; WMS2; ACMICD; ECTOL1; GPHYSD2; FBN

uniprot entry name :

FBN1_HUMAN

reactivity :

Rat

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This FBN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FBN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the Assay: FBN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FBN1. Standards or samples are then added to the microtiter plate wells and FBN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FBN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FBN1 are added to each well to "sandwich" the FBN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FBN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBN1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

281485550

ncbi acc num :

NP_000129.3

ncbi gb acc num :

NM_000138.4

ncbi mol weight :

312,237 Da

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Integrin Cell Surface Interactions Pathway (106110)

ncbi summary :

This gene encodes a member of the fibrillin family. The encoded protein is a large, extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

FBN1: a large, extracellular matrix glycoprotein of the fibrillin family that serves as a structural component of 10-12 nm calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1- containing microfibrils provide long-term force bearing structural support in elastic and nonelastic connective tissue throughout the body. May regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. Defects in this protein are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. Interacts with COL16A1. Protein type: Extracellular matrix; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; microfibril; extracellular region; basement membrane. Molecular Function: protein binding; extracellular matrix structural constituent; calcium ion binding. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; skeletal development; metanephros development. Disease: Ectopia Lentis 1, Isolated, Autosomal Dominant; Mass Syndrome; Weill-marchesani Syndrome 2; Acromicric Dysplasia; Stiff Skin Syndrome; Marfan Syndrome; Geleophysic Dysplasia 2

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage