catalog number :
MBS724333
products type :
ELISA Kit
products full name :
Rat Noradrenaline ELISA Kit
products short name :
Noradrenaline
other names :
NadR; NadR; Transcriptional regulator NadR; NadR
products gene name :
NADR
other gene names :
nadR; nadR; PANA_0620
uniprot entry name :
D4GJH7_PANAM
specificity :
This assay has high sensitivity and excellent specificity for detection of NE. No significant cross-reactivity or interference between NE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NE and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/ml.
products categories :
Neurobiology
products description :
Intended Uses: This NE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NE antibody and an NE-HRP conjugate. The assay sample and buffer are incubated together with NE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE concentration since NE from samples and NE-HRP conjugate compete for the anti-NE antibody binding site. Since the number of sites is limited, as more sites are occupied by NE from the sample, fewer sites are left to bind NE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE concentration in each sample is interpolated from this standard curve.
ncbi acc num :
YP_003518915.1
ncbi gb acc num :
NC_013956.2
ncbi mol weight :
48,465 Da
ncbi pathways :
Metabolic Pathways (146144); Nicotinate And Nicotinamide Metabolism Pathway (146121); Nicotinate And Nicotinamide Metabolism Pathway (400)