Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit | MyBioSource MBS7243258 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit

catalog :

MBS7243258

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7243258

products type :

ELISA Kit

products full name :

Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit

products short name :

Retinal dehydrogenase 1 (ALDH1A1)

other names :

retinal dehydrogenase 1; Retinal dehydrogenase 1; retinal dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; epididymis luminal protein 9; epididymis luminal protein 12; retinaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; epididymis secretory sperm binding protein Li 53e; aldehyde dehydrogenase 1 family, member A1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic

products gene name :

ALDH1A1

other gene names :

ALDH1A1; ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; ALDC; ALDH1; PUMB1; RALDH 1; RalDH1

uniprot entry name :

AL1A1_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of ALDH1A1. No significant cross-reactivity or interference between ALDH1A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALDH1A1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ALDH1A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALDH1A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neuroscience

products description :

Principle of the assay: ALDH1A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDH1A1 antibody and an ALDH1A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH1A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1A1 concentration since ALDH1A1 from samples and ALDH1A1-HRP conjugate compete for the anti-ALDH1A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1A1 from the sample, fewer sites are left to bind ALDH1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1A1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21361176

ncbi acc num :

NP_000680.2

ncbi gb acc num :

NM_000689.4

ncbi mol weight :

54,862 Da

ncbi pathways :

Biological Oxidations Pathway (105698); Ethanol Oxidation Pathway (105715); Fatty Acid Omega Oxidation Pathway (198915); Folate-Alcohol And Cancer Pathway (920980); Metabolic Pathways (132956); Metabolism Pathway (477135); Phase 1 - Functionalization Of Compounds Pathway (105699); Retinol Metabolism Pathway (83020); Retinol Metabolism Pathway (406); Tryptophan Metabolism Pathway (198850)

ncbi summary :

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

uniprot summary :

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family. Protein type: GAPs; Apoptosis; EC 1.2.1.36; GAPs, Ras; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol. Chromosomal Location of Human Ortholog: 9q21.13. Cellular Component: cytoplasm; cytosol. Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity. Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage