ELISA/assay

Rat Aldehyde Dehydrogenase ELISA Kit

MBS724302

48-Strip-Wells

440 USD

ELISA Kit

Rat Aldehyde Dehydrogenase ELISA Kit

[Aldehyde Dehydrogenase]

[aldehyde dehydrogenase, isoform A; Aldehyde dehydrogenase; CG3752 gene product from transcript CG3752-RA; Aldh-PA; Aldh-PB; CG3752-PA; CG3752-PB; acetaldehyde dehydrogenase; Aldehyde dehydrogenase; Aldehyde dehydrogenase; Aldehyde dehydrogenase, isoform A; Aldehyde dehydrogenase, isoform B]

[ALDH]

[Aldh; Aldh; 146084_at; ACDH; ALDH; ALDHA1; CG 3752; CG3752; DmelCG3752; CG3752; Dmel_CG3752]

Q9VLC5_DROME

Rat

This assay has high sensitivity and excellent specificity for detection of ALDH. No significant cross-reactivity or interference between ALDH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALDH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Detection Range: 5.0-100u/L. Sensitivity: 1.0u/l

Neurobiology

Intended Uses: This ALDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALDH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDH antibody and an ALDH-HRP conjugate. The assay sample and buffer are incubated together with ALDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH concentration since ALDH from samples and ALDH-HRP conjugate compete for the anti-ALDH antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH from the sample, fewer sites are left to bind ALDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH concentration in each sample is interpolated from this standard curve.

20129399

NP_609285.1

NM_135441.2

57,019 Da

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48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415