ELISA/assay

Human Pappalysin 2 ELISA Kit

MBS724290

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Pappalysin 2 ELISA Kit

Pappalysin 2

pappalysin-2 isoform 1; Pappalysin-2; pappalysin-2; placenta-specific 3; pregnancy-associated plasma protein E1; pregnancy-associated plasma preproprotein-A2; pappalysin 2; Pregnancy-associated plasma protein A2; PAPP-A2; Pregnancy-associated plasma protein E1; PAPP-E

PAPPA2

PAPPA2; PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2; PLAC3; PAPP-A2; PAPP-E

PAPP2_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PAPPA2. No significant cross-reactivity or interference between PAPPA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAPPA2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

Cardiovascular

Intended Uses This PAPPA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanPAPPA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PAPPA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAPPA2. Standards or samples are then added to the microtiter plate wells and PAPPA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAPPA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PAPPA2 are added to each well to "sandwich” the PAPPA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAPPA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAPPA2 concentration in each sample is interpolated from this standard curve.

116174738

NP_064714.2

NM_020318.2

92,136 Da

Metabolism Of Proteins Pathway (106230); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q25.2. Cellular Component: membrane; extracellular region; intracellular. Molecular Function: metallopeptidase activity; zinc ion binding. Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675