ELISA/assay

Mouse Fibrinogen ELISA Kit

MBS724288

48-Strip-Wells

470 USD

ELISA Kit

Mouse Fibrinogen ELISA Kit

Fibrinogen

Mouse Fibrinogen (Fbg) ELISA Kit; Mouse Fibrinogen ELISA Kit; Fibrinogen; Fibrinogen (Mouse)

fibrinogen; Fibrinogen gamma-B chain; fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide; fibrinogen gamma chain<

Fbg

FGG; FGG; Gamma'

FIBG_BOVIN

Mouse

This assay has high sensitivity and excellent specificity for detection of FBG. No significant cross-reactivity or interference between FBG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Mouse ELISA Kit

Intended Uses: This FBG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FBG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay FBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBG antibody and an FBG-HRP conjugate. The assay sample and buffer are incubated together with FBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBG concentration since FBG from samples and FBG-HRP conjugate compete for the anti-FBG antibody binding site. Since the number of sites is limited, as more sites are occupied by FBG from the sample, fewer sites are left to bind FBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBG concentration in each sample is interpolated from this standard curve.

350

CAA33562.1

P12799

50,244 Da

Common Pathway (827720); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (827717); GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway (827019); Hemostasis Pathway (827694); Integrin AlphaIIb Beta3 Signaling Pathway (827018); Integrin Cell Surface Interactions Pathway (827017); Platelet Aggregation (Plug Formation) Pathway (827712); Platelet Activation, Signaling And Aggregation Pathway (827704)

Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Subunit structure: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain . By similarity. Subcellular location: Secreted. Domain: A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. Post-translational modification: Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Sequence similarities: Contains 1 fibrinogen C-terminal domain.

48-Strip-Wells

470 USD

96-Strip-Wells

675