ELISA/assay

Human Glial Cell Line Derived Neurotrophic Factor ELISA Kit

MBS724250

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Glial Cell Line Derived Neurotrophic Factor

glial cell line-derived neurotrophic factor isoform 4 preproprotein; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor; ATF

GDNF

GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF

GDNF_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Neurobiology

Intended Uses: This GDNF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDNF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GDNF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDNF. Standards or samples are then added to the microtiter plate wells and GDNF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDNF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDNF are added to each well to "sandwich" the GDNF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDNF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDNF concentration in each sample is interpolated from this standard curve.

299473781

NP_001177398.1

NM_001190469.1

18,123 Da

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM1 Interactions Pathway (105697); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915); Spinal Cord Injury Pathway (739007)

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5p13.1-p12. Cellular Component: extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; receptor binding. Biological Process: axon guidance; nervous system development; positive regulation of dopamine secretion; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; neural crest cell migration; metanephros development; neurite development; negative regulation of apoptosis. Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675