ELISA/assay

Rabbit Osteocalcin ELISA Kit

MBS724235

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Osteocalcin ELISA Kit

Osteocalcin

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

OC

BGLAP; BGLAP; OC; BGP; OCN; BGP

OSTCN_HUMAN

Rabbit

This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses This OC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit OC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: OC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OC antibody and an OC-HRP conjugate. The assay sample and buffer are incubated together with OC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OC concentration since OC from samples and OC-HRP conjugate compete for the anti-OC antibody binding site. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.

40316933

NP_954642.1

NM_199173.4

10,963 Da

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); Notch-mediated HES/HEY Network Pathway (169347)

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; regulation of osteoclast differentiation; response to vitamin D; response to ethanol; response to vitamin K; response to mechanical stimulus; response to estrogen stimulus; response to zinc ion; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

48-Strip-Wells

470 USD

96-Strip-Wells

675