ELISA/assay

Human Indoxyl Sulfate ELISA Kit

MBS724219

48-Strip-Wells

440 USD

ELISA Kit

Human Indoxyl Sulfate ELISA Kit

[Indoxyl Sulfate]

[IS]

Human

The microplate is coated with native human thrombin. No cross reactivities to other autoantigens have been found. This ELISA exhibits a specificity of 100% and a sensitivity of 90%.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum. Sensitivity: 1.0 U/ml.

Intended Uses: This is a solid phase enzyme immunoassay employing native human thrombin for the quantitative and qualitative detection of IgA antibodies against thrombin in human serum. For research use only, not for use in diagnostic procedures. Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Thrombin is not a normal constituent of the circulating blood. It is generated by the catalytic cleavage of its plasma precursor, prothrombin (factor II), by the activated Stuart factor (factor Xa). This is the final step of the intrinsic and extrinsic pathways of coagulation. The transformation requires the presence of an activated cofactor, factor Va, released from factor V by thrombin itself, and whose binding to prothrombin accelerates the activity of factor Xa in a non-enzymatic manner. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino-acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Thrombin is, however, more than a simple plasma enzyme. Its properties to stimulate platelets and cause them to expand aggregate and release components of the alpha and dense granules were recognized earlier on.

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415