product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rabbit Keyhole limpet hemocyanin ELISA Kit
catalog :
MBS724211
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS724211
products type :
ELISA Kit
products full name :
Rabbit Keyhole limpet hemocyanin ELISA Kit
products short name :
Keyhole limpet hemocyanin
products gene name :
KLH
reactivity :
Rabbit
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
products categories :
Immunology
products description :
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This KLH ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of KLH in the sample, this KLH ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus KLH concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of KLH utilizes a multiclonal anti-KLH antibody and an KLH-HRP conjugate. The assay sample and buffer are incubated together with KLH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLH concentration since KLH from samples and KLH-HRP conjugate compete for the anti-KLH antibody binding site. Since the number of sites is limited, as more sites are occupied by KLH from the sample, fewer sites are left to bind KLH-HRP conjugate. Standards of known KLH concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of KLH. The KLH concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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