ELISA/assay

Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit

MBS7241942

48-Strip-Wells

440 USD

ELISA Kit

Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit

[Calcitonin gene-related peptide 2 (CALCB)]

[calcitonin gene-related peptide 2; Calcitonin gene-related peptide 2; calcitonin gene-related peptide 2; beta-CGRP; calcitonin 2; beta-type CGRP; calcitonin gene-related peptide II; calcitonin-related polypeptide beta; Beta-type CGRP; Beta-CGRP; Calcitonin gene-related peptide II; CGRP-II]

[CALCB]

[CALCB; CALCB; CALC2; CGRP2; CGRP-II; CALC2; Beta-CGRP; CGRP-II]

CALCB_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CALCB. No significant cross-reactivity or interference between CALCB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALCB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive (Quantitative). Sensitivity: 1.0 pg/mL.

Neurobiology

Intended Uses: This CALCB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CALCB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CALCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CALCB antibody and an CALCB-HRP conjugate. The assay sample and buffer are incubated together with CALCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCB concentration since CALCB from samples and CALCB-HRP conjugate compete for the anti-CALCB antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCB from the sample, fewer sites are left to bind CALCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCB concentration in each sample is interpolated from this standard curve.

9945304

NP_000719.1

NM_000728.3

13,706 Da

Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

CALCB: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Belongs to the calcitonin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.2-p15.1. Cellular Component: extracellular region. Molecular Function: neuropeptide hormone activity. Biological Process: cellular calcium ion homeostasis; signal transduction

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415