Mouse CD81 antigen (CD81) ELISA Kit | MyBioSource MBS7241650 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse CD81 antigen (CD81) ELISA Kit

catalog :

MBS7241650

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7241650

products type :

ELISA Kit

products full name :

Mouse CD81 antigen (CD81) ELISA Kit

products short name :

[CD81 antigen (CD81)]

other names :

[CD81 antigen; CD81 antigen; CD81 antigen; CD 81 antigen; 26 kDa cell surface protein TAPA-1; target of the antiproliferative antibody 1; CD81 antigen (target of antiproliferative antibody 1); Cd81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; CD_antigen: CD81]

products gene name :

[CD81]

other gene names :

[Cd81; Cd81; Tapa1; Tapa1]

uniprot entry name :

CD81_RAT

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of CD81. No significant cross-reactivity or interference between CD81 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD81 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Immunology

products description :

Intended Uses: This CD81 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD81. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CD81 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD81 antibody and an CD81-HRP conjugate. The assay sample and buffer are incubated together with CD81-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD81 concentration since CD81 from samples and CD81-HRP conjugate compete for the anti-CD81 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD81 from the sample, fewer sites are left to bind CD81-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD81 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

6978639

ncbi acc num :

NP_037219.1

ncbi gb acc num :

NM_013087.1

ncbi mol weight :

25,889 Da

ncbi pathways :

Adaptive Immune System Pathway (1010925); B Cell Receptor Signaling Pathway (198486); B Cell Receptor Signaling Pathway (83473); B Cell Receptor Signaling Pathway (492); Hepatitis C Pathway (173970); Hepatitis C Pathway (173907); Immune System Pathway (1010924); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1010951); Malaria Pathway (152662); Malaria Pathway (152657)

ncbi summary :

may play a role in glial scar formation in response to astrocyte injury; may be involved in regulation of cell interactions and cell growth [RGD, Feb 2006]

uniprot summary :

CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family. Protein type: Membrane protein, integral; Cell cycle regulation; Membrane protein, multi-pass. Cellular Component: focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; immunological synapse; vesicle. Molecular Function: protein binding. Biological Process: protein localization; positive regulation of peptidyl-tyrosine phosphorylation; activation of MAPK activity; regulation of growth; positive regulation of 1-phosphatidylinositol 4-kinase activity; positive regulation of cell proliferation; response to wounding; positive regulation of B cell proliferation; phosphatidylinositol biosynthetic process; phosphatidylinositol metabolic process; regulation of cell proliferation

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage