Rat Carnitine palmitoyltransferase I ELISA Kit | MyBioSource MBS724162 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Carnitine palmitoyltransferase I ELISA Kit

catalog :

MBS724162

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS724162

products type :

ELISA Kit

products full name :

Rat Carnitine palmitoyltransferase I ELISA Kit

products short name :

Carnitine palmitoyltransferase I

other names :

Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase 1, liver isoform; carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform; carnitine palmitoyltransferase 1a, liver; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A

products gene name :

CPT-1

other gene names :

Cpt1a; Cpt1a; CPT-Ia; Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L

uniprot entry name :

CPT1A_RAT

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of CPT1. No significant cross-reactivity or interference between CPT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CPT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CPT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: CPT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPT1 antibody and an CPT1-HRP conjugate. The assay sample and buffer are incubated together with CPT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT1 concentration since CPT1 from samples and CPT1-HRP conjugate compete for the anti-CPT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT1 from the sample, fewer sites are left to bind CPT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

146345405

ncbi acc num :

P32198.2

ncbi mol weight :

88,125 Da

ncbi pathways :

AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Beta Oxidation Meta Pathway (198526); Circadian Clock Pathway (1010370); Fatty Acid Beta Oxidation Pathway (219782); Fatty Acid Beta Oxidation 1 Pathway (198531); Fatty Acid Degradation Pathway (83328); Fatty Acid Degradation Pathway (296)

ncbi summary :

enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]

uniprot summary :

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - fatty acid; Membrane protein, integral; EC 2.3.1.21; Membrane protein, multi-pass; Mitochondrial; Transferase. Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; membrane; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane. Molecular Function: identical protein binding; protein binding; carnitine O-palmitoyltransferase activity. Biological Process: response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; triacylglycerol metabolic process; fatty acid beta-oxidation; epithelial cell differentiation; transport; regulation of fatty acid oxidation; regulation of fatty acid beta-oxidation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage