ELISA/assay

Rabbit Apolipoprotein M ELISA Kit

MBS724158

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Apolipoprotein M ELISA Kit

Apolipoprotein M

apolipoprotein M isoform 1; Apolipoprotein M; apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20; apolipoprotein M; Protein G3a

APOM

APOM; APOM; G3a; NG20; apo-M; HSPC336; G3A; NG20; HSPC336; Apo-M; ApoM

APOM_HUMAN

Rabbit

This assay has high sensitivity and excellent specificity for detection of APOM. No significant cross-reactivity or interference between APOM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APOM and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This APOM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit APOM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: APOM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APOM antibody and an APOM-HRP conjugate. The assay sample and buffer are incubated together with APOM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOM concentration since APOM from samples and APOM-HRP conjugate compete for the anti-APOM antibody binding site. Since the number of sites is limited, as more sites are occupied by APOM from the sample, fewer sites are left to bind APOM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOM concentration in each sample is interpolated from this standard curve.

22091452

NP_061974.2

NM_019101.2

13,051 Da

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p21.33. Cellular Component: integral to plasma membrane. Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding. Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; lipoprotein metabolic process; cholesterol efflux

48-Strip-Wells

470 USD

96-Strip-Wells

675