ELISA/assay

Mouse Beclin 1 ELISA Kit

MBS724152

48-Strip-Wells

470 USD

ELISA Kit

Mouse Beclin 1 ELISA Kit

Beclin 1

beclin 1; Beclin-1; beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197

Beclin 1

BECN1; BECN1; ATG6; VPS30; beclin1; GT197

BECN1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of BECN-1. No significant cross-reactivity or interference between BECN-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BECN-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenat. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Cancer

Intended Uses: This BECN-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BECN-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BECN-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BECN-1 antibody and an BECN-1-HRP conjugate. The assay sample and buffer are incubated together with BECN-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BECN-1 concentration since BECN-1 from samples and BECN-1-HRP conjugate compete for the anti-BECN-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BECN-1 from the sample, fewer sites are left to bind BECN-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BECN-1 concentration in each sample is interpolated from this standard curve.

4680381

AAD27650.1

51,896 Da

Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Protein type: Autophagy; Membrane protein, peripheral; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; dendrite; cytoplasmic membrane-bound vesicle; trans-Golgi network; phagocytic vesicle; nucleus; endosome. Molecular Function: protein binding; phosphoinositide 3-kinase binding. Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; response to hypoxia; mitochondrion degradation; cellular defense response; neuron development; positive regulation of macroautophagy; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation

48-Strip-Wells

470 USD

96-Strip-Wells

675