ELISA/assay

Human 1, 25-dihydroxyvitamin D3 ELISA Kit

MBS724145

48-Strip-Wells

470 USD

ELISA Kit

Human 1, 25-dihydroxyvitamin D3 ELISA Kit

1, 25-dihydroxyvitamin D3

Human 1,25-dihydroxyvitamin D3 (1,25OHVD3) ELISA Kit; Human 1, 25-dihydroxyvitamin D3 ELISA Kit; 1, 25-dihydroxyvitamin D3; 1, 25-dihydroxyvitamin D3 (Human)

DVD/DHVD 3

Human

This assay has high sensitivity and excellent specificity for detection of 1,25-OHVD3. No significant cross-reactivity or interference between 1,25-OHVD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 1,25-OHVD3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Human ELISA Kit

Intended Uses: This 1,25-OHVD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 1,25-OHVD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay 1,25-OHVD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-1,25-OHVD3 antibody and an 1,25-OHVD3-HRP conjugate. The assay sample and buffer are incubated together with 1,25-OHVD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25-OHVD3 concentration since 1,25-OHVD3 from samples and 1,25-OHVD3-HRP conjugate compete for the anti-1,25-OHVD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25-OHVD3 from the sample, fewer sites are left to bind 1,25-OHVD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25-OHVD3 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675