ELISA/assay

Human Serum Deprivation Response Protein ELISA Kit

MBS724139

48-Strip-Wells

470 USD

ELISA Kit

Human Serum Deprivation Response Protein ELISA Kit

Serum Deprivation Response Protein

serum deprivation-response protein; Serum deprivation-response protein; serum deprivation-response protein; phosphatidylserine binding protein; phosphatidylserine-binding protein; serum deprivation response; Cavin-2; PS-p68; Phosphatidylserine-binding protein

SDPR

SDPR; SDPR; SDR; CAVIN2; PS-p68; cavin-2

SDPR_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of SDRP. No significant cross-reactivity or interference between SDRP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDRP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SDRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SDRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: SDRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDRP antibody and an SDRP-HRP conjugate. The assay sample and buffer are incubated together with SDRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDRP concentration since SDRP from samples and SDRP-HRP conjugate compete for the anti-SDRP antibody binding site. Since the number of sites is limited, as more sites are occupied by SDRP from the sample, fewer sites are left to bind SDRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDRP concentration in each sample is interpolated from this standard curve.

4759082

NP_004648.1

NM_004657.5

47,173 Da

This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.[provided by RefSeq, Sep 2009]

SDPR: May play a role in targeting PRKCA to caveolae. Belongs to the PTRF/SDPR family. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 2q32-q33. Cellular Component: cytoplasm; caveola; cytosol. Molecular Function: protein binding; protein kinase C binding; phosphatidylserine binding; phospholipid binding

48-Strip-Wells

470 USD

96-Strip-Wells

675