catalog number :
MBS724100
products type :
ELISA Kit
products full name :
Human Bone Alkaline Phosphatase ELISA Kit
products short name :
[Bone Alkaline Phosphatase]
other names :
[bap; BAP; BAP; Bap]
products gene name :
[BALP]
other gene names :
[bap; Dsecap; Dsec_GM10423; GM10423]
uniprot entry name :
B2ZIB7_DROSE
specificity :
This assay has high sensitivity and excellent specificity for detection of BAG5. No significant cross-reactivity or interference between BAG5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAG5 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.
products description :
Intended Uses: This BAG5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BAG5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BAG5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BAG5 antibody and an BAG5-HRP conjugate. The assay sample and buffer are incubated together with BAG5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAG5 concentration since BAG5 from samples and BAG5-HRP conjugate compete for the anti-BAG5 antibody binding site. Since the number of sites is limited, as more sites are occupied by BAG5 from the sample, fewer sites are left to bind BAG5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAG5 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
EDW55325.1
ncbi mol weight :
42,112 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)
size5 :
5x96-Strip-Wells-(Competitive)
size6 :
5x96-Strip-Wells-(Sandwich)
size7 :
10x96-Strip-Wells-(Competitive)
size8 :
10x96-Strip-Wells-(Sandwich)