ELISA/assay

Human Ly6/PLAUR domain containing protein 3 (LYPD3) ELISA Kit

MBS7240935

48-Strip-Wells

470 USD

ELISA Kit

Human Ly6/PLAUR domain containing protein 3 (LYPD3) ELISA Kit

Ly6/PLAUR domain containing protein 3 (LYPD3)

ly6/PLAUR domain-containing protein 3; Ly6/PLAUR domain-containing protein 3; ly6/PLAUR domain-containing protein 3; MIG-C4; 2310061G07Rik; matrigel-induced gene C4 protein; GPI-anchored metastasis-associated protein homolog; GPI-anchored metastasis-associated protein C4.4A homolog; LY6/PLAUR domain containing 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4

LYPD3; LYPD3; C4.4A; C4.4A; UNQ491/PRO1007; MIG-C4

LYPD3_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of LYPD3. No significant cross-reactivity or interference between LYPD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LYPD3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This LYPD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LYPD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: LYPD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LYPD3 antibody and an LYPD3-HRP conjugate. The assay sample and buffer are incubated together with LYPD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LYPD3 concentration since LYPD3 from samples and LYPD3-HRP conjugate compete for the anti-LYPD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by LYPD3 from the sample, fewer sites are left to bind LYPD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LYPD3 concentration in each sample is interpolated from this standard curve.

93004088

NP_055215.2

NM_014400.2

35,971 Da

LYPD3: Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 19q13.31. Cellular Component: extracellular space; anchored to plasma membrane; integral to membrane. Molecular Function: laminin binding. Biological Process: cell-matrix adhesion

48-Strip-Wells

470 USD

96-Strip-Wells

675