product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Endothelial nitric oxide synthase 3 ELISA Kit
catalog :
MBS724085
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS724085
products type :
ELISA Kit
products full name :
Mouse Endothelial nitric oxide synthase 3 ELISA Kit
products short name :
Endothelial nitric oxide synthase 3
other names :
endothelial nitric oxide synthase 3 isoform 3, partial; Endothelial nitric oxide synthase 3 isoform 3; Endothelial nitric oxide synthase 3 isoform 3
products gene name :
ENOS-3
other gene names :
NOS3
uniprot entry name :
H9CZQ8_CHICK
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of eNOS3. No significant cross-reactivity or interference between eNOS3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between eNOS3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This eNOS3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse eNOS3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: eNOS3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-eNOS3 antibody and an eNOS3-HRP conjugate. The assay sample and buffer are incubated together with eNOS3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the eNOS3 concentration since eNOS3 from samples and eNOS3-HRP conjugate compete for the anti-eNOS3 antibody binding site. Since the number of sites is limited, as more sites are occupied by eNOS3 from the sample, fewer sites are left to bind eNOS3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS3 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
379975450
ncbi acc num :
AFD20686.1
ncbi mol weight :
51,109 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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