ELISA/assay

Mouse Choline acetyltransferase ELISA Kit

MBS724080

48-Strip-Wells

470 USD

ELISA Kit

Mouse Choline acetyltransferase ELISA Kit

Choline acetyltransferase

choline acetyltransferase, partial; Choline O-acetyltransferase; choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; choline O-acetyltransferase

ChAT

CHAT; CHAT; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase

CLAT_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ChAT. No significant cross-reactivity or interference between ChAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ChAT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This ChAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ChAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: ChAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ChAT antibody and an ChAT-HRP conjugate. The assay sample and buffer are incubated together with ChAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ChAT concentration since ChAT from samples and ChAT-HRP conjugate compete for the anti-ChAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ChAT from the sample, fewer sites are left to bind ChAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ChAT concentration in each sample is interpolated from this standard curve.

553229

AAC37590.1

70,394 Da

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Neuronal System Pathway (106513)

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. Protein type: Acetyltransferase; EC 2.3.1.6; Lipid Metabolism - glycerophospholipid. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol. Molecular Function: choline O-acetyltransferase activity. Biological Process: synaptic transmission; rhythmic behavior; rhythmic excitation; muscle development; neurotransmitter secretion; phospholipid metabolic process; glycerophospholipid biosynthetic process; dendrite development; phosphatidylcholine biosynthetic process; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process. Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

48-Strip-Wells

470 USD

96-Strip-Wells

675