Human Aldehyde dehydrogenase family 1 member A3 ELISA Kit | MyBioSource MBS724053 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Aldehyde dehydrogenase family 1 member A3 ELISA Kit

catalog :

MBS724053

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS724053

products type :

ELISA Kit

products full name :

Human Aldehyde dehydrogenase family 1 member A3 ELISA Kit

products short name :

Aldehyde dehydrogenase family 1 member A3

other names :

aldehyde dehydrogenase family 1 member A3 isoform 1; Aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3; aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3

products gene name :

ALDH1A3

other gene names :

ALDH1A3; ALDH1A3; ALDH6; MCOP8; RALDH3; ALDH1A6; ALDH6; RALDH-3; RalDH3

uniprot entry name :

AL1A3_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ADP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

153266822

ncbi acc num :

NP_000684.2

ncbi gb acc num :

NM_000693.3

ncbi mol weight :

56,108 Da

ncbi pathways :

Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Histidine Metabolism Pathway (82958); Histidine Metabolism Pathway (324); Metabolic Pathways (132956); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

ncbi summary :

This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

uniprot summary :

ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.5; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - phenylalanine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: cytoplasm; cytosol. Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity. Biological Process: inner ear morphogenesis; retinal metabolic process; embryonic eye morphogenesis; righting reflex; positive regulation of apoptosis; retinol metabolic process; olfactory pit development; optic cup morphogenesis involved in camera-type eye development; retinoic acid metabolic process; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development. Disease: Microphthalmia, Isolated 8

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage