ELISA/assay

Rat copeptin ELISA Kit

MBS724037

48-Strip-Wells

470 USD

ELISA Kit

Rat copeptin ELISA Kit

copeptin

copeptin

copeptin

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses: This CPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CPP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPP antibody and an CPP-HRP conjugate. The assay sample and buffer are incubated together with CPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPP concentration since CPP from samples and CPP-HRP conjugate compete for the anti-CPP antibody binding site. Since the number of sites is limited, as more sites are occupied by CPP from the sample, fewer sites are left to bind CPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPP concentration in each sample is interpolated from this standard curve.

225612

1307200C

48-Strip-Wells

470 USD

96-Strip-Wells

675