ELISA/assay

Mouse Chemokine C-C-Motif Ligand 1 ELISA Kit

MBS724009

48-Strip-Wells

470 USD

ELISA Kit

Mouse Chemokine C-C-Motif Ligand 1 ELISA Kit

Chemokine C-C-Motif Ligand 1

C-C motif chemokine 1; C-C motif chemokine 1; C-C motif chemokine 1; P500; TCA3; CCR8 ligand; SIS-epsilon; T-cell activation protein 3; small inducible cytokine A1; small-inducible cytokine A1; chemokine (C-C motif) ligand 1; P500; SIS-epsilon; Small-inducible cytokine A1; T-cell activation protein 3; TCA-3; TCA3

CCL1

Ccl1; Ccl1; I-309; Scya1; Tca-3; BF534335; Scya1; Tca3; TCA-3; TCA3

CCL1_MOUSE

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . Intended Uses: This CCL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CCL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . Principle of the Assay: CCL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-1 antibody and an CCL-1-HRP conjugate. The assay sample and buffer are incubated together with CCL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-1 concentration since CCL-1 from samples and CCL-1-HRP conjugate compete for the anti-CCL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-1 from the sample, fewer sites are left to bind CCL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-1 concentration in each sample is interpolated from this standard curve.

6755424

NP_035459.1

NM_011329.3

9,836 Da

Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460)

CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family. Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity; cytokine activity. Biological Process: immune response; chemotaxis

48-Strip-Wells

470 USD

96-Strip-Wells

675